Alpha-Factor Mating Pheromone Epigenetics High-Throughput/Automated Isolation The Chromatin Wash Buffer II

The Chromatin Wash Buffer II is designed to perform the second wash of the Protein A bead/antibody/chromatin complexes in the Zymo-Spin ChIP Kit

is suitable for hybridization

For automation scripts and support

mitochondrial) from fresh

SDS-PAGE and other sensitive applications

Alpha-Factor Mating Pheromone Epigenetics High-Throughput/Automated Isolation The Chromatin Wash Buffer IIWhen yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes, affectionately termed "schmooing". These alterations prepare the yeast cells for mating and fusion to form stable diploids. The a alpha diploids are not responsive to mating pheromone